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Escherichia coli K-12が生産するイソアスパラギナーゼのα-アスパルチルジペプチダーゼとしての同定
https://pu-toyama.repo.nii.ac.jp/records/199
https://pu-toyama.repo.nii.ac.jp/records/19952562a23-8a0b-4bd2-bdb5-7ed05faf0099
名前 / ファイル | ライセンス | アクション |
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KJ00004259886.pdf (663.0 kB)
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Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-02-24 | |||||
タイトル | ||||||
タイトル | Escherichia coli K-12が生産するイソアスパラギナーゼのα-アスパルチルジペプチダーゼとしての同定 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Identification of Isoasparaginase from Escherichia coli K-12 as α-aspartyl dipeptidase | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | アスパルテーム | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | イソアスパラギナーゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | α-アスパルチルジペプチダーゼ(PepE) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | アスパルチルアミノペプチダーゼ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Escherichia coli | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Aspartame | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | isoasparaginase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | α-aspartyl dipeptidase (PepE) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | aspartyl aminopeptidase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Escherichia coli | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10358595 | |||||
著者 |
浅野, 泰久
× 浅野, 泰久× 水口, 直之× 米田, 英伸× 吉良, 郁夫× 横関, 健三× ASANO, Yasuhisa× MIZUGUCHI, Naoyuki× KOMEDA, Hidenobu× KIRA, Ikuo× YOKOZEKI, Kenzo |
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著者所属(日) | ||||||
値 | 生物工学研究センター | |||||
著者所属(日) | ||||||
値 | 生物工学研究センター | |||||
著者所属(日) | ||||||
値 | 生物工学研究センター | |||||
著者所属(日) | ||||||
値 | 生物工学研究センター | |||||
著者所属(日) | ||||||
値 | 味の素(株) | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Biotechnology Research Center | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Biotechnology Research Center | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Biotechnology Research Center | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | On leave from Ajinomoto Co. Ltd. | |||||
著者所属(英) | ||||||
言語 | en | |||||
値 | Ajinomoto Co. Ltd. | |||||
抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Escherichia coli K-12(TPU6303)の無細胞抽出液よりイソアスパラギナーゼを精製し、そのN末端アミノ酸配列を決定した。TSK-GEL Octyl-80Tsを用いてHPLCで精製されたフラクションαおよびフラクションβの分子量は、SDS-PAGEにより、それぞれ約25,000および27,000と算出された。フラクションαのN末端アミノ酸配列を20残基決定し、E.coliのリボース-5-リン酸イソメラーゼAと同定した。フラクションβのN末端アミノ酸配列を18残基決定し、α-アスパルチルジペプチダーゼ(PepE)(アスパルチルアミノペプチダーゼ、EC 3.4.11.21)と同定した。以上より、E. coliに検出されたイソアスパラギナーゼはα-アスパルチルジペプチダーゼと同一酵素であることが明らかになった。 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Isoasparaginase was purified from the cell-free extract of Escherichia coli K-12 (TPU6303) and its N-terminal amino acid sequence was determined. The molecular weight of the fraction α and fraction β, which were separated by HPLC on a TSK-GEL Octyl-80Ts column, was calculated to be about 25,000 and 27,000 by the SDS-PAGE. Twenty residues in the amino-terminal region of fraction α were identical to 20 residues of ribose-5-phosphate isomerase A from E. coli. Eighteen residues in the amino-terminal region of fraction β were identical to 20 residues of α-aspartyl dipeptidase (peptidase B) from E. coli and Salmonella typhimurium Isoasparaginase was judged to be equal to aspartyl dipeptidase (PepE) (aspartyl aminopeptidase, EC 3.4.11.21). Therefore, isoasparaginase activity found in E. coli was identified as aspartyl dipeptidase. | |||||
書誌情報 |
富山県立大学紀要 en : Bulletin of Toyama Prefectural University 巻 14, p. 96-103, 発行日 2004-03-31 |
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表示順 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 9 | |||||
アクセション番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | KJ00004259886 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09167633 |